Bagaço de cana-de-açúcar e bagaço de sisal como substratos indutores para a produção de endoglucanase por actinobacteria isolada de solo de cultura de sisal

Dayse Batista dos Santos, Aline Simões da Rocha Bispo, Rodrigo Pires do Nascimento, Márcia Luciana Cazetta

Resumo


Resumo: Uma linhagem de actinobactéria, isolada de solo de cultura de sisal (Agave sisalana
Perrine) no semiárido baiano, denominada ARA-01, foi selecionada como uma linhagem promissora
para a produção de endoglucanase em substratos agroindustriais. Bagaço de cana-de-açúcar e
bagaço de sisal foram empregados como fonte de carbono e água de maceração de milho como fonte
de nitrogênio, em fermentação submersa do tipo batelada. Os resultados mostraram que o meio
contendo 2,4 % de bagaço de cana-de-açúcar e 1,3 % de água de maceração de milho levaram a
uma produção de 230 U.L-1 de endoglucanase, no segundo dia de fermentação. Com o bagaço de
sisal foi obtida produção de 630 U.L-1 de endoglucanase na condição de 1,6 % de bagaço de sisal e
1,5 % de água de maceração de milho, após 3 dias de fermentação. Estes resultados mostraram que
a actinobacteria ARA-01 e os substratos agroindustriais utilizados apresentaram bom potencial para
produção de endoglucanase.
Palavras chave: Enzimas microbianas, Substratos agroindustriais, Bactéria.


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